Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-16
... efficiency of this method seems to depend on the particular mRNA under study . In the best cases ( e.g. , chicken lysozyme mRNA [ Land et al . 1981 ] ) , full - length clones of cDNA can be obtained with high efficiency ; other mRNAs ...
... efficiency of this method seems to depend on the particular mRNA under study . In the best cases ( e.g. , chicken lysozyme mRNA [ Land et al . 1981 ] ) , full - length clones of cDNA can be obtained with high efficiency ; other mRNAs ...
Page 8-23
... efficiency of cloning , the number of homopolymeric residues on the plasmid and the cDNA should be approximately equal , with approxi- mately 100 dA / dT residues or 20 dC / dG residues being added to each end of the DNAs ( Peacock et ...
... efficiency of cloning , the number of homopolymeric residues on the plasmid and the cDNA should be approximately equal , with approxi- mately 100 dA / dT residues or 20 dC / dG residues being added to each end of the DNAs ( Peacock et ...
Page 15-78
... efficiency of transfection in ung bacteria of bacteriophage M13 DNA that contains uracil in one strand is greatly ... efficiency of the extension reaction . In addition , the concentration of each of the four dNTPs in the reaction should ...
... efficiency of transfection in ung bacteria of bacteriophage M13 DNA that contains uracil in one strand is greatly ... efficiency of the extension reaction . In addition , the concentration of each of the four dNTPs in the reaction should ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml