Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-48
... all in one preparation . cDNAs that have been cloned using subtracted cDNA probes include the murine J immunoglobulin chain ( Mather et al . 1981 ) and the murine T - cell receptor ( Hedrick et al . 1984 ) . A slightly different ...
... all in one preparation . cDNAs that have been cloned using subtracted cDNA probes include the murine J immunoglobulin chain ( Mather et al . 1981 ) and the murine T - cell receptor ( Hedrick et al . 1984 ) . A slightly different ...
Page 11-13
... et al . ( 1985 ) Ullrich et al . ( 1986 ) " Eightfold degenerate . bTwofold degenerate . the gene of interest and forms hybrids that are stable. human platelet - derived growth factor A - chain human protein kinase C bovine chromogranin ...
... et al . ( 1985 ) Ullrich et al . ( 1986 ) " Eightfold degenerate . bTwofold degenerate . the gene of interest and forms hybrids that are stable. human platelet - derived growth factor A - chain human protein kinase C bovine chromogranin ...
Page 12-3
... et al . 1988 ; Vinson et al . 1988 ) . The DNA - binding domains of these proteins include such structural motifs as zinc fingers ( Miller et al . 1985 ; for review , see Evans and Hollenberg 1988 ) , leucine zippers ( Landschulz et al ...
... et al . 1988 ; Vinson et al . 1988 ) . The DNA - binding domains of these proteins include such structural motifs as zinc fingers ( Miller et al . 1985 ; for review , see Evans and Hollenberg 1988 ) , leucine zippers ( Landschulz et al ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml