Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 8-55
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
Page 9-22
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
Page 13-98
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml