Molecular Cloning: A Laboratory Manual, Book 2 |
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Page vi
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
Page xiii
... Ethidium Bromide Solutions 6.16 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA ...
... Ethidium Bromide Solutions 6.16 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA ...
Page 15-88
... ethidium bromide gradients ( see Chapter 1 , pages 1.42-1.45 ) . Approximately 40 μg of closed circular DNA will be required . The DNA should be freshly purified to minimize the proportion of linear and relaxed circular forms of the ...
... ethidium bromide gradients ( see Chapter 1 , pages 1.42-1.45 ) . Approximately 40 μg of closed circular DNA will be required . The DNA should be freshly purified to minimize the proportion of linear and relaxed circular forms of the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml