Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 54
Page 10-56
... exonuclease activity of bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as ...
... exonuclease activity of bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as ...
Page 13-39
... Exonuclease III 1. Digest 10 μg of recombinant bacteriophage M13 replicative form DNA or recombinant phagemid DNA with two restriction enzymes that cleave in the polycloning site between the primer - binding site of the vector and the ...
... Exonuclease III 1. Digest 10 μg of recombinant bacteriophage M13 replicative form DNA or recombinant phagemid DNA with two restriction enzymes that cleave in the polycloning site between the primer - binding site of the vector and the ...
Page 15-18
... exonuclease III is by far the best : • The exonucleolytic reaction proceeds at a remarkably uniform and predict- able rate . This means that it is possible to isolate not only nested deletions , but also groups of deletions whose ...
... exonuclease III is by far the best : • The exonucleolytic reaction proceeds at a remarkably uniform and predict- able rate . This means that it is possible to isolate not only nested deletions , but also groups of deletions whose ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml