Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiv
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 SELECTION OF POLY ( A ) ...
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 SELECTION OF POLY ( A ) ...
Page 9-16
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer · 10 mM Tris Cl ( pH 8.0 ) 0.1 M EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 ...
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer · 10 mM Tris Cl ( pH 8.0 ) 0.1 M EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 ...
Page 9-17
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml