Molecular Cloning: A Laboratory Manual, Book 2 |
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Page ix
... EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and ...
... EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and ...
Page xxvii
... Extracts 16.59 16.59 Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID ...
... Extracts 16.59 16.59 Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID ...
Page 8-58
... extracts are much cheaper to make than to buy . If you plan to construct more than one or two cDNA libraries , it would be worthwhile making a large batch of packaging extract ( see Chapter 2 , pages 2.98- 2.107 ) . However , Gigapack ...
... extracts are much cheaper to make than to buy . If you plan to construct more than one or two cDNA libraries , it would be worthwhile making a large batch of packaging extract ( see Chapter 2 , pages 2.98- 2.107 ) . However , Gigapack ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml