Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 95
Page 9-39
... filter in transfer buffer for at least 5 minutes . Using a clean scalpel blade , cut a corner from the nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies ...
... filter in transfer buffer for at least 5 minutes . Using a clean scalpel blade , cut a corner from the nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies ...
Page 12-17
... filters . Do not move the filter once contact is made with the plate . The easiest way to place the filter on the plate without trapping air bubbles is to hold it by its edges , bending it slightly so that the middle of the filter makes ...
... filters . Do not move the filter once contact is made with the plate . The easiest way to place the filter on the plate without trapping air bubbles is to hold it by its edges , bending it slightly so that the middle of the filter makes ...
Page 12-21
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml