Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 70
Page 8-62
... final volume of 48 μl Add 40 units of avian reverse transcriptase and mix as described above . Incubate for 2 hours at 42 ° C . Preparations of reverse transcriptase purified from particles of avian retroviruses usually contain between ...
... final volume of 48 μl Add 40 units of avian reverse transcriptase and mix as described above . Incubate for 2 hours at 42 ° C . Preparations of reverse transcriptase purified from particles of avian retroviruses usually contain between ...
Page 10-26
... final length of the probe , the specific activity of the 5 ' terminus of the probe will be considerably higher than that of the 3 ' terminus . In general , this asymmetry does not matter when the probes are to be used in northern or ...
... final length of the probe , the specific activity of the 5 ' terminus of the probe will be considerably higher than that of the 3 ' terminus . In general , this asymmetry does not matter when the probes are to be used in northern or ...
Page 13-84
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml