Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 54
Page 9-49
... concentrations of SDS , which will cause the milk proteins to precipitate . If background hybridization is a problem , NP - 40 may be added to the hybridization solution to a final concentration of 1 % . BLOTTO should not be used as a ...
... concentrations of SDS , which will cause the milk proteins to precipitate . If background hybridization is a problem , NP - 40 may be added to the hybridization solution to a final concentration of 1 % . BLOTTO should not be used as a ...
Page 13-84
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
Page 14-20
... final concentration 100 μg / ml ) may be substituted for gelatin in the reverse transcription reaction . The amount of MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase ...
... final concentration 100 μg / ml ) may be substituted for gelatin in the reverse transcription reaction . The amount of MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml