Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 34
Page 97
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and λgt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and λgt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
Page 9-7
... foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors were the source of most of the cloned segments of mam- malian genomic DNA isolated before 1983 , and some of these libraries are used ...
... foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors were the source of most of the cloned segments of mam- malian genomic DNA isolated before 1983 , and some of these libraries are used ...
Page 15-3
... foreign DNA sequences cloned in plasmid vectors . If the restriction map or , better yet , the complete nucleotide sequence of the foreign DNA is known , it may be possible to construct a set of deletion mutants by digestion with the ...
... foreign DNA sequences cloned in plasmid vectors . If the restriction map or , better yet , the complete nucleotide sequence of the foreign DNA is known , it may be possible to construct a set of deletion mutants by digestion with the ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml