Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Apparatus Contents xiii 6.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Apparatus Contents xiii 6.
Page xiv
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
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... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml