Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-37
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cI gene product and a high frequency of ...
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cI gene product and a high frequency of ...
Page 11-14
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
Page 12-6
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml