Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 92
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from which they were originally derived . The feasibility of using libraries in this way was established in the late 1970s ...
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from which they were originally derived . The feasibility of using libraries in this way was established in the late 1970s ...
Page 11-7
... genome , are counted only once . Only approximate values of C are known for genomes that have not been sequenced completely . The following estimates are taken from Laird ( 1971 ) . Genome Size a Complexity Escherichia coli 4.0 × 106 ...
... genome , are counted only once . Only approximate values of C are known for genomes that have not been sequenced completely . The following estimates are taken from Laird ( 1971 ) . Genome Size a Complexity Escherichia coli 4.0 × 106 ...
Page 12-6
... genomic DNA expression library , the proportion of recombinants capable of expressing a fusion protein encoded by a gene whose coding region is 1.5 kb in length is predicted to be : E. coli Yeast Genome size ( kb ) 4.0 × 103 Proportion ...
... genomic DNA expression library , the proportion of recombinants capable of expressing a fusion protein encoded by a gene whose coding region is 1.5 kb in length is predicted to be : E. coli Yeast Genome size ( kb ) 4.0 × 103 Proportion ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml