Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 92
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
Page 9-31
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
Page 12-6
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml