Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 42
Page 12-17
... 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours at 37 ° C . If the library is not to be screened in duplicate , the original filter can be left on the plate for 10-12 ...
... 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours at 37 ° C . If the library is not to be screened in duplicate , the original filter can be left on the plate for 10-12 ...
Page 12-23
... 37 ° C . Colonies containing expression vectors carrying the bacteriophage A PR promoter should be grown at 30 ° C ... hours at 4 ° C in an inverted position . 2. Label a dry , sterile nitrocellulose filter ( Millipore HAWP or equivalent ) ...
... 37 ° C . Colonies containing expression vectors carrying the bacteriophage A PR promoter should be grown at 30 ° C ... hours at 4 ° C in an inverted position . 2. Label a dry , sterile nitrocellulose filter ( Millipore HAWP or equivalent ) ...
Page 15-65
... C , 5 minutes at room temperature , and then 2 hours at 37 ° C . The low temperature optimizes initiation of DNA synthesis from the 3 ' terminus , and the subsequent incubation at 37 ° C improves the efficiency of the extension reaction ...
... C , 5 minutes at room temperature , and then 2 hours at 37 ° C . The low temperature optimizes initiation of DNA synthesis from the 3 ' terminus , and the subsequent incubation at 37 ° C improves the efficiency of the extension reaction ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml