Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 95
Page 9-47
... hybridization Although the choice depends to a large extent on personal preference , we offer the following guidelines for choosing among the various methods available . 1. Hybridization reactions in 50 % formamide at 42 ° C are less ...
... hybridization Although the choice depends to a large extent on personal preference , we offer the following guidelines for choosing among the various methods available . 1. Hybridization reactions in 50 % formamide at 42 ° C are less ...
Page 9-48
... hybridization are un- known . When using probes that have the capacity to self - anneal ( e.g. , nick - translated double - stranded DNA ) , the following rule of thumb is useful : Allow the hybridization to proceed for a time ...
... hybridization are un- known . When using probes that have the capacity to self - anneal ( e.g. , nick - translated double - stranded DNA ) , the following rule of thumb is useful : Allow the hybridization to proceed for a time ...
Page 9-50
... hybridization signal is particularly noticeable when oligonucleotides or probes less than 100 nucleotides in length are used . 7. In the presence of 10 % dextran sulfate or 10 % polyethylene glycol , the rate of hybridization is ...
... hybridization signal is particularly noticeable when oligonucleotides or probes less than 100 nucleotides in length are used . 7. In the presence of 10 % dextran sulfate or 10 % polyethylene glycol , the rate of hybridization is ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml