Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-44
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
Page 12-16
... infected . In each tube , mix 0.1 ml of the plating bacteria with 0.1 ml of SM containing 3 × 10 * pfu ( 90 - mm plates ) or 105 pfu ( 150 - mm plates ) of the bacteriophage › expression library . Incubate the infected bacteria for 20 ...
... infected . In each tube , mix 0.1 ml of the plating bacteria with 0.1 ml of SM containing 3 × 10 * pfu ( 90 - mm plates ) or 105 pfu ( 150 - mm plates ) of the bacteriophage › expression library . Incubate the infected bacteria for 20 ...
Page 13-76
... infected culture grown for not more than 4.5 hours Plaque - purify recombinant bacteriophage ; make sure that single - stranded DNA is prepared from an infected culture grown for not more than 4.5 hours Titrate primer or use an ...
... infected culture grown for not more than 4.5 hours Plaque - purify recombinant bacteriophage ; make sure that single - stranded DNA is prepared from an infected culture grown for not more than 4.5 hours Titrate primer or use an ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml