Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 76
Page 8-6
... interest , or of mRNA that has been partially fragmented by limited alkaline hydrolysis and end - labeled by phosphor- ylation . As a good approximation , the mRNA sequences of interest will be represented in both the probe and the ...
... interest , or of mRNA that has been partially fragmented by limited alkaline hydrolysis and end - labeled by phosphor- ylation . As a good approximation , the mRNA sequences of interest will be represented in both the probe and the ...
Page 8-7
... interest are present at even lower levels ( 1 molecule / cell is not unusual [ Toole et al . 1984 ; Wood et al . 1984 ] ) . Further- more , it is often necessary to clone cDNAs from populations of mRNAs isolated from tissues that ...
... interest are present at even lower levels ( 1 molecule / cell is not unusual [ Toole et al . 1984 ; Wood et al . 1984 ] ) . Further- more , it is often necessary to clone cDNAs from populations of mRNAs isolated from tissues that ...
Page 10-41
... interest cells not expressing mRNA ( s ) of interest poly ( A ) RNA annem m Generate radioactive probe by reverse transcription of the entire mRNA population using oligo ( dT ) or short digonucleotides of random sequence as primers ...
... interest cells not expressing mRNA ( s ) of interest poly ( A ) RNA annem m Generate radioactive probe by reverse transcription of the entire mRNA population using oligo ( dT ) or short digonucleotides of random sequence as primers ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml