Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiv
53 ISOLATION OF INTACT DNA FROM YEAST 6 . 55 RESTRICTION ENZYME
DIGESTION OF DNA IN AGAROSE BLOCKS 6 . 57 Markers for Pulsed - field Gel
Electrophoresis 6 . 58 References 6 . 60 Extraction , Purification , and Analysis of
...
53 ISOLATION OF INTACT DNA FROM YEAST 6 . 55 RESTRICTION ENZYME
DIGESTION OF DNA IN AGAROSE BLOCKS 6 . 57 Markers for Pulsed - field Gel
Electrophoresis 6 . 58 References 6 . 60 Extraction , Purification , and Analysis of
...
Page 57
Several manufacturers sell enzyme isolated from a strain of E . coli lysogenic for
a bacteriophage i that carries the gene ( polA ) that codes for the holoenzyme of
E . coli DNA polymerase I ( Murray and Kelley 1979 ) . Most commercial suppliers
...
Several manufacturers sell enzyme isolated from a strain of E . coli lysogenic for
a bacteriophage i that carries the gene ( polA ) that codes for the holoenzyme of
E . coli DNA polymerase I ( Murray and Kelley 1979 ) . Most commercial suppliers
...
Page 15
Validation of Clones Isolated by Immunological Screening The identification of
immunoreactive clones from expression libraries cannot be taken as proof that
the cDNA of interest has been isolated . The specificity of antibodies is never ...
Validation of Clones Isolated by Immunological Screening The identification of
immunoreactive clones from expression libraries cannot be taken as proof that
the cDNA of interest has been isolated . The specificity of antibodies is never ...
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LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
TEST LIGATION 3 29 | 3 |
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 11 |
FRAGMENT OF E coli DNA POLYMERASE I 13 59 | 14 |
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Common terms and phrases
acid activity agarose aliquots allow amount amplification appropriate approximately bacteriophage bacteriophage T4 base buffer carried cDNA cells centrifugation Chapter chromatography cleaved clones colonies concentration constructed containing deletions denatured described determine digestion DNA polymerase dNTPs double-stranded efficiency electrophoresis et al ethanol expression Figure filters fragment gene genomic hybridization increase Incubate inserted interest isolated labeled length libraries ligation linear linkers method microfuge minutes mixture molecules mRNA mutagenesis mutagenic mutations Nucleic nucleotides obtained oligonucleotide original plaques plasmid plasmid DNA plate pool position possible prepared presence primer probe problem protein purified radiolabeled reaction recombinant region remove restriction enzyme room temperature samples screening sequence single single-stranded solution specific step Store strand synthesis target DNA target sequence template termini tion transfer transformants tube units usually vector volume