Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 89
Page xiv
... ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
... ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
Page 8-57
... isolated from a strain of E. coli lysogenic for a bacteriophage λ that carries the gene ( polA ) that codes for the ... isolated from a strain of E. coli that expresses the RI restriction and modification genes from a multicopy plasmid ...
... isolated from a strain of E. coli lysogenic for a bacteriophage λ that carries the gene ( polA ) that codes for the ... isolated from a strain of E. coli that expresses the RI restriction and modification genes from a multicopy plasmid ...
Page 12-15
... isolated . The specificity of antibodies is never absolute and the epitope ( s ) recognized on the protein is usually undefined . Further tests are therefore required to validate the identity of the initial isolates . In decreasing ...
... isolated . The specificity of antibodies is never absolute and the epitope ( s ) recognized on the protein is usually undefined . Further tests are therefore required to validate the identity of the initial isolates . In decreasing ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml