Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-52
... labeled DNA fragments that can be used as size markers during gel electrophoresis . Because DNA fragments are labeled in proportion to their molar concen- trations and not their sizes , both small and large fragments in a restric- tion ...
... labeled DNA fragments that can be used as size markers during gel electrophoresis . Because DNA fragments are labeled in proportion to their molar concen- trations and not their sizes , both small and large fragments in a restric- tion ...
Page 11-15
... LABELING OF GUESSMERS Oligonucleotides less than 70 nucleotides long are usually labeled by phos- phorylation catalyzed by bacteriophage T4 polynucleotide kinase ( see pages 11.31-11.32 ) or by primer extension using the Klenow fragment ...
... LABELING OF GUESSMERS Oligonucleotides less than 70 nucleotides long are usually labeled by phos- phorylation catalyzed by bacteriophage T4 polynucleotide kinase ( see pages 11.31-11.32 ) or by primer extension using the Klenow fragment ...
Page 13-100
... labeled with polynucleotide kinase and [ y - 32PJATP ) . Probable causes Suggested solutions Reproduced with permission , from Maxam and Gilbert ( 1980. 11. An extraneous band across all four lad- ders at one position in a sequencing ...
... labeled with polynucleotide kinase and [ y - 32PJATP ) . Probable causes Suggested solutions Reproduced with permission , from Maxam and Gilbert ( 1980. 11. An extraneous band across all four lad- ders at one position in a sequencing ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml