Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-26
... length of the newly synthesized chains . • Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
... length of the newly synthesized chains . • Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
Page 11-8
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
Page 15-89
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml