Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 99
Page 8-38
... libraries of bacterial lysogens carrying recombinant Agt11 genomes were induced by heating and screened for the subsequent production of foreign antigens . Propagation of recombinant bac- teriophages as lysogens appeared to offer ...
... libraries of bacterial lysogens carrying recombinant Agt11 genomes were induced by heating and screened for the subsequent production of foreign antigens . Propagation of recombinant bac- teriophages as lysogens appeared to offer ...
Page 92
... libraries of eukaryotic genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ( Clarke and Carbon 1976 ) , genomic DNA libraries are now increasingly viewed as matrices that can ...
... libraries of eukaryotic genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ( Clarke and Carbon 1976 ) , genomic DNA libraries are now increasingly viewed as matrices that can ...
Page 12-6
... libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For prokaryotic organisms , whose genomes contain few introns , there is little to be gained by constructing cDNA libraries ...
... libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For prokaryotic organisms , whose genomes contain few introns , there is little to be gained by constructing cDNA libraries ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml