Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-5
... linear molecules ( Melgar and Goldthwait 1968 ) . These unit- length linear molecules are purified to remove circular plasmid DNA , recircularized in the presence of a synthetic linker containing a restriction enzyme cleavage site that ...
... linear molecules ( Melgar and Goldthwait 1968 ) . These unit- length linear molecules are purified to remove circular plasmid DNA , recircularized in the presence of a synthetic linker containing a restriction enzyme cleavage site that ...
Page 15-9
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
Page 15-89
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml