Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 14
Page 8-3
... lines or tissues . In addition , some workers have found ways to increase the concentration of the relevant mRNA by using drugs to select cell lines that overexpress particular proteins . For example , Luskey et al . ( 1983 ) describe ...
... lines or tissues . In addition , some workers have found ways to increase the concentration of the relevant mRNA by using drugs to select cell lines that overexpress particular proteins . For example , Luskey et al . ( 1983 ) describe ...
Page 9-18
... line . With the vacuum line closed , slowly lower the pipette to the bottom of the organic phase . Wait until the viscous thread of aqueous material detaches from the pipette , and then carefully open the vacuum line and gently withdraw ...
... line . With the vacuum line closed , slowly lower the pipette to the bottom of the organic phase . Wait until the viscous thread of aqueous material detaches from the pipette , and then carefully open the vacuum line and gently withdraw ...
Page 15-66
... line across the strip about 2.5 cm from one end , and make two small marks about one inch apart on the line . b . Spot 0.5 μl of each of the aliquots set aside in steps 1 and 2 at the marks , and place the strip vertically in a ...
... line across the strip about 2.5 cm from one end , and make two small marks about one inch apart on the line . b . Spot 0.5 μl of each of the aliquots set aside in steps 1 and 2 at the marks , and place the strip vertically in a ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml