Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-7
... linkers are then ligated directly to these blunt ends . An alternative strategy is to use linkers that carry protruding termini complementary to the termini generated by the restriction enzyme ( Barany 1985a , b ) . Hexa- meric linkers ...
... linkers are then ligated directly to these blunt ends . An alternative strategy is to use linkers that carry protruding termini complementary to the termini generated by the restriction enzyme ( Barany 1985a , b ) . Hexa- meric linkers ...
Page 15-33
... linkers are then inserted at the site of the nick or gap , and a novel method is used to identify and clone target DNA fragments in which exactly 8 base pairs have been removed and replaced with the synthetic DNA linker . There are also ...
... linkers are then inserted at the site of the nick or gap , and a novel method is used to identify and clone target DNA fragments in which exactly 8 base pairs have been removed and replaced with the synthetic DNA linker . There are also ...
Page 15-86
... linkers must be used in place of single - stranded linkers . ) • The restriction site that is to be inserted as part of the hexameric linker should not occur at any other place in the target DNA or the plasmid vector . • The choice of ...
... linkers must be used in place of single - stranded linkers . ) • The restriction site that is to be inserted as part of the hexameric linker should not occur at any other place in the target DNA or the plasmid vector . • The choice of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml