Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-38
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
Page 12-38
... Lysogens The isolation of recombinant bacteriophages encoding fusion proteins that bind to double - stranded ... lysogenic for recombinant bacteriophage Agt11 . Usually 10-70 % of the colonies tested are lysogens . 7. Inoculate 2 ...
... Lysogens The isolation of recombinant bacteriophages encoding fusion proteins that bind to double - stranded ... lysogenic for recombinant bacteriophage Agt11 . Usually 10-70 % of the colonies tested are lysogens . 7. Inoculate 2 ...
Page 12-39
... lysogens are induced . 10. Transfer the cultures to a water bath equilibrated to 44 ° C . Incubate the cultures for ... lysogenic cultures to two microfuge tubes . Immediately centrifuge the tubes at 12,000g for 30 seconds at 4 ° C in a ...
... lysogens are induced . 10. Transfer the cultures to a water bath equilibrated to 44 ° C . Incubate the cultures for ... lysogenic cultures to two microfuge tubes . Immediately centrifuge the tubes at 12,000g for 30 seconds at 4 ° C in a ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml