Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 39
Page 8-6
... mRNAs represent a sufficiently high proportion of the starting population . Low - abundance mRNAs mRNAs that represent less than 0.5 % of the total mRNA population of the cell are classified as " low - abundance " or " rare " mRNAs ...
... mRNAs represent a sufficiently high proportion of the starting population . Low - abundance mRNAs mRNAs that represent less than 0.5 % of the total mRNA population of the cell are classified as " low - abundance " or " rare " mRNAs ...
Page 8-8
... mRNA of the cell . The modal size of the mRNA population extracted from most types of mammalian cells is approximately 1.8 kb , and mRNAs smaller in size than 700 bases or larger than 4 kb can be enriched at least tenfold by a single ...
... mRNA of the cell . The modal size of the mRNA population extracted from most types of mammalian cells is approximately 1.8 kb , and mRNAs smaller in size than 700 bases or larger than 4 kb can be enriched at least tenfold by a single ...
Page 8-48
... mRNA preparations , i.e. , when most species of mRNA are represented equally in the two preparations and a small proportion ( < 2 % ) of the mRNAs are not present at all in one preparation . cDNAs that have been cloned using subtracted ...
... mRNA preparations , i.e. , when most species of mRNA are represented equally in the two preparations and a small proportion ( < 2 % ) of the mRNAs are not present at all in one preparation . cDNAs that have been cloned using subtracted ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml