Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-32
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
Page 1-19
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
Page 1-36
... mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA- dependent RNA polymerases TATA box , 16.6 upstream promoter elements , 16.6 Pronase ...
... mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA- dependent RNA polymerases TATA box , 16.6 upstream promoter elements , 16.6 Pronase ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml