Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiv
... MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for Pulsed - field Gel Electrophoresis 6.58 References 6.60 7 Extraction , Purification , and Analysis of ...
... MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for Pulsed - field Gel Electrophoresis 6.58 References 6.60 7 Extraction , Purification , and Analysis of ...
Page xxvi
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
Page 1-19
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml