Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-11
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
Page 14-10
... method or , more commonly , by the Sanger dideoxy - mediated chain- termination method ( see Chapter 13 ) . Three types of polymerase chain reaction are used to generate templates for sequencing by the Sanger method : • A conventional ...
... method or , more commonly , by the Sanger dideoxy - mediated chain- termination method ( see Chapter 13 ) . Three types of polymerase chain reaction are used to generate templates for sequencing by the Sanger method : • A conventional ...
Page 1-6
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml