Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 14
Page xii
... METHYLASE 5.15 dcm METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli 5.15 M.EcoRI METHYLASE 5.16 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA ...
... METHYLASE 5.15 dcm METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli 5.15 M.EcoRI METHYLASE 5.16 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA ...
Page 8-39
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
Page 1-38
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage X , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66-8.67 hsdM , genetic marker in E ...
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage X , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66-8.67 hsdM , genetic marker in E ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml