Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-75
... mg / ml in dimethylformamide ) and 4 μl of a solution of IPTG ( 200 mg / ml ) for each 90 - mm petri dish . Incubate the plates for 8-10 hours at 42 ° C . Plaque formation with ZAP : Grow E. coli strain BB4 in NZCYM medium containing ...
... mg / ml in dimethylformamide ) and 4 μl of a solution of IPTG ( 200 mg / ml ) for each 90 - mm petri dish . Incubate the plates for 8-10 hours at 42 ° C . Plaque formation with ZAP : Grow E. coli strain BB4 in NZCYM medium containing ...
Page 9-49
... mg / ml in 4 × SSPE or 4 × SSC and stored at 4 ° C . It is used as a blocking agent at a concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate ...
... mg / ml in 4 × SSPE or 4 × SSC and stored at 4 ° C . It is used as a blocking agent at a concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate ...
Page 12-29
... mg / ml . 2. To 50 μl of the diluted IgG , add 4 μl of 125I ( sodium 125 iodide , 10 mCi / ml ) . 3. Add 15 μl of chloramine T ( 2 mg / ml in 50 mM sodium phosphate ( pH 7.5 ] ) . 4. Incubate for 90 seconds at room temperature . 5. Add ...
... mg / ml . 2. To 50 μl of the diluted IgG , add 4 μl of 125I ( sodium 125 iodide , 10 mCi / ml ) . 3. Add 15 μl of chloramine T ( 2 mg / ml in 50 mM sodium phosphate ( pH 7.5 ] ) . 4. Incubate for 90 seconds at room temperature . 5. Add ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml