Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 75
Page 13-40
... microfuge tube or well of the microtiter plate containing the S1 reaction mixture . To the remainder of the DNA solution , add 150 units of exonuclease III per picomole of recessed 3 ' termini . Vortex briefly to mix the contents of the ...
... microfuge tube or well of the microtiter plate containing the S1 reaction mixture . To the remainder of the DNA solution , add 150 units of exonuclease III per picomole of recessed 3 ' termini . Vortex briefly to mix the contents of the ...
Page 14-14
... microfuge , disposable gloves , supplies , and sets of pipetting devices used only to handle polymerase chain ... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the ...
... microfuge , disposable gloves , supplies , and sets of pipetting devices used only to handle polymerase chain ... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the ...
Page 15-42
... microfuge tube , and adjust the volume to 180 μl with TE ( pH 7.6 ) . 2. Add 20 μl of 10x ligase buffer ... microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ...
... microfuge tube , and adjust the volume to 180 μl with TE ( pH 7.6 ) . 2. Add 20 μl of 10x ligase buffer ... microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml