Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 57
Page 11-36
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
Page 13-61
... microfuge tube , and then add 4 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
... microfuge tube , and then add 4 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
Page 14-14
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml