Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 84
Page 10-64
... minutes at 4 ° C in a microfuge . 2. Dissolve the DNA in a minimum volume of 10 mM Tris · Cl ( pH 8.3 ) . 3. Add ... minutes at 37 ° C ; add a second aliquot of CIP , and continue incubation for a further 30 minutes . To dephosphorylate ...
... minutes at 4 ° C in a microfuge . 2. Dissolve the DNA in a minimum volume of 10 mM Tris · Cl ( pH 8.3 ) . 3. Add ... minutes at 37 ° C ; add a second aliquot of CIP , and continue incubation for a further 30 minutes . To dephosphorylate ...
Page 13-86
... minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ° C . Add 4 ...
... minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ° C . Add 4 ...
Page 15-24
... minutes , 3.0 minutes , 4.5 minutes , etc. 3. Add 36 μl of the appropriate dilution of BAL 31 ( see step 6 , page 15.23 ) to the reaction mixture prepared in step 1. Quickly mix the enzyme by tapping the side of the tube , and then ...
... minutes , 3.0 minutes , 4.5 minutes , etc. 3. Add 36 μl of the appropriate dilution of BAL 31 ( see step 6 , page 15.23 ) to the reaction mixture prepared in step 1. Quickly mix the enzyme by tapping the side of the tube , and then ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml