Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
Page 15-27
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
Page 15-39
... molecules , which migrate much slower through agarose gels than closed circular DNAs of the same size . There should be no increase in the ratio of relaxed circular molecules to super- helical molecules in the control samples . Pool the ...
... molecules , which migrate much slower through agarose gels than closed circular DNAs of the same size . There should be no increase in the ratio of relaxed circular molecules to super- helical molecules in the control samples . Pool the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml