Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 34
Page 10-10
... nick translation are sensitive to contaminants in agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ...
... nick translation are sensitive to contaminants in agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ...
Page 10-11
... nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower ... Nick Translation 10 8 Alternative Protocol for Nick Translation 10 11.
... nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower ... Nick Translation 10 8 Alternative Protocol for Nick Translation 10 11.
Page 15-33
... nicks or gaps . The com- plementary DNA strand is then cleaved opposite the nick or gap by digestion with nuclease S1 . This usually results in the removal of 0-30 nucleotides from each DNA strand at the site of the nick and generates a ...
... nicks or gaps . The com- plementary DNA strand is then cleaved opposite the nick or gap by digestion with nuclease S1 . This usually results in the removal of 0-30 nucleotides from each DNA strand at the site of the nick and generates a ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml