Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-38
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS ... nitrocellulose filter on the surface of a 9.38 Analysis and Cloning of Eukaryotic Genomic ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS ... nitrocellulose filter on the surface of a 9.38 Analysis and Cloning of Eukaryotic Genomic ...
Page 9-39
... nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies enormously . If the filter is not saturated after floating for several minutes on water , it should be ...
... nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies enormously . If the filter is not saturated after floating for several minutes on water , it should be ...
Page 9-41
... nitrocellulose filters . 3. While the gel is soaking , cut and wet two pieces of nitrocellulose ( 0.45- micron pore size ) , first in water and then in 10 × SSC . Cut one corner from each filter . To retain small fragments of DNA ...
... nitrocellulose filters . 3. While the gel is soaking , cut and wet two pieces of nitrocellulose ( 0.45- micron pore size ) , first in water and then in 10 × SSC . Cut one corner from each filter . To retain small fragments of DNA ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml