Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 54
Page 8-65
... nuclease - S1 buffer . 10x Nuclease - S1 buffer 2 M NaCl 0.5 M sodium acetate ( pH 4.5 ) 10 mM ZnSO , 5 % glycerol f . Add 5 units of nuclease S1 to one of the two tubes containing the first strand of cDNA and to one containing the ...
... nuclease - S1 buffer . 10x Nuclease - S1 buffer 2 M NaCl 0.5 M sodium acetate ( pH 4.5 ) 10 mM ZnSO , 5 % glycerol f . Add 5 units of nuclease S1 to one of the two tubes containing the first strand of cDNA and to one containing the ...
Page 15-7
... nuclease S1 . Synthetic phosphor- ylated linkers are then ligated directly to these blunt ends . An alternative ... nuclease BAL 31 or a combination of exonuclease III and nuclease S1 or mung - bean nuclease , and then re- circularizing ...
... nuclease S1 . Synthetic phosphor- ylated linkers are then ligated directly to these blunt ends . An alternative ... nuclease BAL 31 or a combination of exonuclease III and nuclease S1 or mung - bean nuclease , and then re- circularizing ...
Page 15-39
... nuclease S1 ) . Superhelical DNA is under torsional strain and may therefore be susceptible to cleavage at particular sites by nuclease S1 . Digestion by nuclease S1 at these sites would generate linear molecules whose termini would map ...
... nuclease S1 ) . Superhelical DNA is under torsional strain and may therefore be susceptible to cleavage at particular sites by nuclease S1 . Digestion by nuclease S1 at these sites would generate linear molecules whose termini would map ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml