Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-7
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
Page 11-8
... nucleotides in length would be reduced 5-7.5 ° C by internal mismatches . This is sufficient leeway to allow easy discrimination between perfectly matched and internally mismatched hybrids formed by short ( < 20 nucleotides ) ...
... nucleotides in length would be reduced 5-7.5 ° C by internal mismatches . This is sufficient leeway to allow easy discrimination between perfectly matched and internally mismatched hybrids formed by short ( < 20 nucleotides ) ...
Page 15-55
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml