Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-21
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
Page 11-30
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate the solution containing the ... OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate the solution containing the ... OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
Page 11-31
... oligonucleotide to high specific activity . Labeling of different amounts of oligonucleotide can easily be achieved by increasing or decreasing the size of the reaction , keeping the concentrations of all components constant . 1. Set up ...
... oligonucleotide to high specific activity . Labeling of different amounts of oligonucleotide can easily be achieved by increasing or decreasing the size of the reaction , keeping the concentrations of all components constant . 1. Set up ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml