Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 35
Page 9-29
... pellet of nucleic acid with 70 % ethanol and recentrifuge for 2 minutes . Remove the ethanol by careful aspiration , and allow the pellet to dry at room temperature . Redissolve the pellet in TE ( pH 7.6 ) at a concentration of 1 μg ...
... pellet of nucleic acid with 70 % ethanol and recentrifuge for 2 minutes . Remove the ethanol by careful aspiration , and allow the pellet to dry at room temperature . Redissolve the pellet in TE ( pH 7.6 ) at a concentration of 1 μg ...
Page 13-83
... pellet after each wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and ...
... pellet after each wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and ...
Page 13-98
... pellet , and poor vacuum during the lyophilization steps are common causes of this problem . Hydrazine has undergone oxidation . Residual salt from ethanol precipitation of end - labeled DNA is carried over into the sequencing reactions ...
... pellet , and poor vacuum during the lyophilization steps are common causes of this problem . Hydrazine has undergone oxidation . Residual salt from ethanol precipitation of end - labeled DNA is carried over into the sequencing reactions ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml