Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 74
Page 8-73
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
Page 12-16
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
Page 15-70
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml