Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 88
Page vi
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
Page 15-5
... plasmid DNA . The two methods differ in the techniques used to achieve this random cleavage . 1. Partial digestion with pancreatic DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a ...
... plasmid DNA . The two methods differ in the techniques used to achieve this random cleavage . 1. Partial digestion with pancreatic DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a ...
Page 15-9
... DNA mixture to the second tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip ... plasmid DNA with an excess ( 5 units ) of a restriction enzyme that cleaves the DNA only once . The resulting linear DNA ...
... DNA mixture to the second tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip ... plasmid DNA with an excess ( 5 units ) of a restriction enzyme that cleaves the DNA only once . The resulting linear DNA ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml