Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 80
Page 11-24
... plates , clean the plates with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution : Handle the KOH and the KOH / methanol solutions with great care . Use gloves and a face protector . Then ...
... plates , clean the plates with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution : Handle the KOH and the KOH / methanol solutions with great care . Use gloves and a face protector . Then ...
Page 12-17
... plate . Wetting action then pulls the filter onto the plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours ...
... plate . Wetting action then pulls the filter onto the plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours ...
Page 13-49
... plates and reduces the possibility that the gel will tear when it is removed from the mold when electrophoresis is completed . To siliconize a plate , lay the plate on a pad of paper in a chemical hood , and pour a small quantity of ...
... plates and reduces the possibility that the gel will tear when it is removed from the mold when electrophoresis is completed . To siliconize a plate , lay the plate on a pad of paper in a chemical hood , and pour a small quantity of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml