Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
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Page 11-9
... position of codon ט כ AG טכ AG 3rd position of codon ( 3 ' terminus ) U с A G บ บ บ Phe UUC Phe UCU Ser UCC Ser UAU Tyr UAC Tyr UGU Cys UGC Cys U UUA Leu UUG Leu UCA Ser UCG Ser UAA UAG Stop ( Ochre ) Stop ( Amber ) UGA UGG Trp Stop ...
... position of codon ט כ AG טכ AG 3rd position of codon ( 3 ' terminus ) U с A G บ บ บ Phe UUC Phe UCU Ser UCC Ser UAU Tyr UAC Tyr UGU Cys UGC Cys U UUA Leu UUG Leu UCA Ser UCG Ser UAA UAG Stop ( Ochre ) Stop ( Amber ) UGA UGG Trp Stop ...
Page 11-11
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30-70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . Hybridization with oligonucleotides ...
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30-70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . Hybridization with oligonucleotides ...
Page 11-17
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml