Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 43
Page 8-65
... Precipitate the eluted cDNA by adding 0.1 volume of 3 M sodium acetate ( pH 5.2 ) and 2 volumes of ethanol . Store the sample at room temperature for at least 15 minutes . Recover the precipitated DNA by centrifugation at 12,000g for 15 ...
... Precipitate the eluted cDNA by adding 0.1 volume of 3 M sodium acetate ( pH 5.2 ) and 2 volumes of ethanol . Store the sample at room temperature for at least 15 minutes . Recover the precipitated DNA by centrifugation at 12,000g for 15 ...
Page 10-65
... precipitate to dry completely at room temperature before dissolving it in TE ( pH 7.6 ) at a concentration of more ... precipitates from solution if its concentration exceeds 5-10 mм . ii . Until recently , CIP was supplied as a ...
... precipitate to dry completely at room temperature before dissolving it in TE ( pH 7.6 ) at a concentration of more ... precipitates from solution if its concentration exceeds 5-10 mм . ii . Until recently , CIP was supplied as a ...
Page 11-35
... precipitate radiolabeled nucleic acids , including oligonucleotides , from labeling reactions . The nucleic acids are precipitated from aqueous solution with the cationic detergent cetylpyridinium bromide ( CPB ) . The detergent is then ...
... precipitate radiolabeled nucleic acids , including oligonucleotides , from labeling reactions . The nucleic acids are precipitated from aqueous solution with the cationic detergent cetylpyridinium bromide ( CPB ) . The detergent is then ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml