Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-48
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
Page 9-53
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
Page 10-18
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml