Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 99
Page xviii
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page 10-18
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
Page 10-27
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from ... probes has led to Preparation of Radiolabeled DNA and RNA Probes 10.27 Plasmid Vectors.
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from ... probes has led to Preparation of Radiolabeled DNA and RNA Probes 10.27 Plasmid Vectors.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml